气相色谱法检测橄榄油中反式脂肪酸含量的方法学研究
Methodological Study on the Detection of Trans Fatty Acid Content in Olive Oil by Gas Chromatography
彭万才 1唐顺之 1李遥 1魏劭恒 1李佳俐 1许文东1
作者信息
- 1. 中药制药过程技术与新药创制国家工程研究中心,广州白云山汉方现代药业有限公司,广东省药用脂质重点实验室,广东广州 510240
- 折叠
摘要
目的:建立气相色谱法检测橄榄油中 10 种反式脂肪酸的方法并进行方法学验证.方法:样品经甲酯化后,以Supelco SP-2560 毛细管柱为分析柱,经程序升温和氢火焰离子化检测器进行检测.结果:橄榄油中10种反式脂肪酸能实现良好分离,未见其他成分干扰,方法的检测限和定量限、精密度和回收率、耐用性、专属性均良好.结论:该方法准确可靠、简单易行,可用于橄榄油中反式脂肪酸含量检测.
Abstract
Objective:To establish a gas chromatography method for the determination of 10 trans-fatty acids in olive oil and to verify the method.Method:After methylation,the Supelco SP-2560 capillary column was used as the analysis column,and the samples were detected by temperature program and hydrogen flame ionization detector.Result:The 10 kinds of trans fatty acids in olive oil could be separated well without interference from other components.The detection limit and quantitation limit,precision and recovery rate,durability and specificity of the method were all good.Conclusion:The method is accurate,reliable,simple and suitable for the determination of trans fatty acids in olive oil.
关键词
气相色谱法/橄榄油/反式脂肪酸Key words
gas chromatography/olive oil/trans fatty acids引用本文复制引用
出版年
2024
NETL
NSTL
万方数据