Determination of Total Amino Acids and Optimization of Ultrasonic Breaking Technology in Pollen of Pinus yunnanensis
Objective:To optimize the method of the ultrasonic breaking and establish determination method of total amino acids inpollen of Pinus yunnanensis.Method:Aspartic acid was used as the control,the content of total amino acids in pollen of Pinus yunnanensis was determined by spectrophotometry method.Single factor test and orthogonal test were used to investigate the best experimental conditions for breaking walls.Result:The standard curve equation of A=92.143C-0.060 3,r=0.999 5,and a good linear correlation was found in range of 2 μg·mL-1 to 10 μg·mL-1 within the mass concentration of aspartic concentration.The average recovery was 99.9%,relative standard deviation was 1.85%(n=6).The best process for ultrasonically broken pine pollen was as follows:material volume 5.0 g,ultrasonic power 700 W,ultrasonic time 40 min,and the wall breaking rate could reach 98.0%.Conclusion:The established method for the determination of total amino acid content in pollen of Pinus yunnanensis is accurate.The optimal wall breaking method is stable and feasible.
pollen of Pinus yunnanensistotal amino acidspectrophotometrycontent determinationultrasonic wall breaking
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