Analysis of Volatile Components and Antioxidant Effects of the Different Ethanol Concentrations of Gymnadenia conopsea(L.)R.Br.
In order to investigate the effects of different ethanol concentrations on the types of volatile components and antioxidant capacity of Gymnadenia conopsea(L.)R.Br.,this experiment used headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)to determine the volatile components of Gymnadenia conopsea(L.)R.Br.ethanol extracts and Gymnadenia conopsea(L.)R.Br.raw materials at concentrations of 75%and 95%.Enzyme linked immunosorbent assay(ELISA)was used to evaluate the total antioxidant capacity(T-AOC),DPPH radical and superoxide anion radical(O2-·)scavenging ability of the two Gymnadenia conopsea(L.)R.Br.ethanol extracts.The results showed that a total of 82 volatile substances were detected in the raw materials and two extracts.Among them,95%of the volatile components in the ethanol extract of Gymnadenia conopsea(L.)R.Br.are the most diverse,with 56 types;there are 16 common volatile components and 4 different components in the ethanol extracts of two types of Gymnadenia conopsea(L.)R.Br.;the T-AOC and DPPH free radical scavenging ability of 95%Gymnadenia conopsea(L.)R.Br.ethanol extract was significantly higher than that of 75%Gymnadenia conopsea(L.)R.Br.ethanol extract,but the O2-·scavenging rate was significantly lower than that of 75%Gymnadenia conopsea(L.)R.Br.ethanol extract;the volatile components of extracts such as cresol,ethyl hexadecanoate,styrene,and octanol were significantly positively correlated with T-AOC and DPPH radical scavenging ability,and significantly negatively correlated with O2-·scavenging ability.This study can provide reference for the development of volatile functional components in the Gymnadenia conopsea(L.)R.Br..
万方数据
维普
