Application of SPE-UHPLC-MS/MS in the Determination of Chlorpheniramine Residues in Pork
王丹1
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作者信息
1. 秦安县食品药品检验检测中心,甘肃秦安 741600
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摘要
本研究采用固相萃取-超高效液相色谱-串联质谱法(Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry,SPE-UHPLC-MS/MS)对猪肉中氯苯胺灵的残留进行检测.通过优化气相色谱-质谱条件,建立了氯苯胺灵的标准曲线,结果表明,氯苯胺灵在0.50～100.00 μg·L-1 线性关系良好,相关系数为 0.996 4.平均加标回收率为85.25%～92.75%,相对标准偏差为 1.83%～3.07%.该方法回收率好、精密度高,适用于猪肉中氯苯胺灵的快速、准确检测.
Abstract
This study used solid phase extraction ultra high performance liquid chromatography tandem mass spectrometry(SPE-UPLC-MS/MS)to detect residues of chlorpheniramine in pork.By optimizing the gas chromatography-mass spectrometry conditions,a standard curve for chlorpheniramine was established.The results showed that chlorpheniramine had a good linear relationship between 0.50 μg·L-1 and 100.00 μg·L-1,with a correlation coefficient of 0.996 4.The average recovery rate of spiked samples is 85.25%to 92.75%,and the relative standard deviation is 1.83%～3.07%.This method has good recovery rate and high precision,and is suitable for rapid and accurate detection of chlorpheniramine in pork.
关键词
固相萃取-超高效液相色谱-串联质谱法/猪肉/氯苯胺灵
Key words
solid phase extraction-ultra performance liquid chromatography tandem mass spectrometry/pork/chlorpheniramine
维普
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