This experiment optimized the method for determining ligustrazine in Shanxi aged vinegar by high-performance liquid chromatography in appendix B of GB/T 19777-2013.The pH value of the solution system,the amount of trichloromethane,and the centrifugation conditions during the sample processing were studied through single factor experiments and orthogonal experiments.Finally,it was determined to use sodium hydroxide solution(200 g·L-1)to adjust the pH to 11.0,extract twice with 5 mL of chloroform,and centrifuge at 6 000 r·min-1 for 5 min.The best results were obtained under the treatment conditions,with a standard recovery rate ranging from 90.9%to 93.8%and a relative standard deviation of 0.47%to 1.19%,all of which can meet the requirements for the determination of ligustrazine in Shanxi aged vinegar.
维普
万方数据