Determination of Pantothenic Acid in Food by Semi-Preparative Monolithic Column Liquid Chromatography
Objective:To establish.an analytical method for the determination of pantothenic acid content in food using semi-preparative monolithic column liquid chromatography.Method:Pantothenic acid in the sample is extracted with hot water,using a solution of potassium dihydrogen phosphate and acetonitrile as the mobile phase,with a flow rate of 2 mL·min-1,an injection volume of 500 μL,a column temperature of 30℃,and a detection wavelength of 200 nm.The solution is purified on a Merck semi-preparative monolithic column,and the solution is collected at the elution peak of pantothenic acid.The collected liquid is then quantitatively analyzed on a reverse-phase C18 column.Result:Pantothenic acid exhibits good linearity within the concentration range of 0.001～0.032 μg·L-1,with a correlation coefficient of 0.999 9.The limits of detection is 0.025 mg/100 g,and limits of quantification is 0.08 mg/100 g.The relative standard deviation of the pantothenic acid determination results in food is between 2.4%and 3.6%.The recovery rates of spiked samples are greater than 90%.Conclusion:For foods containing complex matrices such as proteins,peptides,and starch,this semi-preparative monolithic column liquid chromatography method demonstrates good consistency in detection,with separation,precision,and accuracy all meeting the necessary standards.It effectively addresses issues such as difficult peak separation,unstable detection results and poor parallelism under complex matrices and impurity interference.
high performance liquid chromatographypantothenic acidsemi-preparative monolithic column
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维普
