Determination of Florfenicol Residues in Pork by QuEChERS-HPLC-MS/MS
Objective:To establish a method for the determination of florfenicol residue in pork by QuEChERS cleanup combined with high performance liquid chromatography tandem mass spectrometry.Method:The sample was crushed and homogenized and then extracted with acetonitrile.The extract was cleaned up by QuEChERS and separated by C18 column.Acetonitrile and ultrapure water were used as mobile phases for elution.Full scan and multiple reaction monitoring mode were used for detection and internal standard method for quantification.Result:Florfenicol had a good linear relationship in the concentration range of 0～50 μg·L-1,with correlation coefficient R2≥0.999,limit of quantification of 0.02 μg·kg-1,and limit of detection of 0.01 μg·kg-1.At the three spiked concentration levels of 10 μg·kg-1,20 μg·kg-1,and 50 μg·kg-1,the recoveries were 98.2%～108.5%,and the RSD were 3.24%～5.75%.Conclusion:This method has simple pretreatment operation,high accuracy and sensitivity,and meets the requirements for the detection of florfenicol residues in pork.
florfenicolQuEChERShigh performance liquid chromatography tandem mass spectrometrypork
万方数据
维普
