Establish an analytical method for the determination of residues of four quinolone drugs,including enrofloxacin,ciprofloxacin,ofloxacin,and sarafloxacin,in pork using ultra-high performance liquid chromatography-mass spectrometry.The sample was extracted with EDTA Mcllvaine buffer solution,purified with HLB solid-phase extraction column,concentrated with nitrogen blowing,and then dissolved in 1 mL formic acid aqueous solution(0.1%)for measurement on the machine.The results showed that there was a good linear relationship between the residual components of the four quinolone drugs in the range of 2.5～100.0 μg·L-1.The detection limit of the method was 1.0～2.5 μg·kg-1,and the quantification limit was 3.0～8.0 μg·kg-1.The recovery rate was in the range of 90.0%～109.2%,and the relative standard deviation(RSD)was 1.01%～5.74%.It is suitable for the detection of four quinolone drug residues in pork.
关键词
超高效液相色谱-质谱联用法/猪肉/喹诺酮
Key words
ultra high performance liquid chromatography-mass spectrometry/pork/quinolone
维普
万方数据