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蛋白粉中蛋白质含量测定的不确定度评估

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应用《食品安全国家标准 食品中蛋白质的测定》(GB 5009.5-2016)第一法凯氏定氮法测定蛋白粉中蛋白质的含量,参考《化学分析中不确定度的评估指南》(CNAS-GL006:2019)及《测量不确定度评定与表示》(JJF 1059.1-2012)进行蛋白质测量的不确定度评价.结果表明,蛋白粉中蛋白质的含量为86.90 g/100 g,扩展不确定度为 2.64 g/100 g,因此蛋白质含量测定结果报告为(86.90±2.64)g/100 g.影响蛋白粉中蛋白质测定不确定度的主要因素为定氮过程,其次是样品的称量、前处理、测量及盐酸标准溶液滴定浓度.
Uncertainty Evaluation for Determination of Protein in Protein Powder
The protein content in protein powder is determined using the first method of GB 5009.5-2016 automatic Kjeldahl nitrogen analyzer,with reference to CNAS-GL006:2019 and JJF 1059.1-2012 for protein measurement uncertainty evaluation.The results showed that the protein content in protein powder was determined to be 86.90 g/100 g using the Kjeldahl nitrogen analyzer method,with an expanded uncertainty of 2.64 g/100 g.Therefore,the protein content determination result was reported as(86.90±2.64)g/100 g.The main factor affecting the uncertainty of protein determination in protein powder is the nitrogen determination process,followed by sample weighing,pretreatment,sample measurement,and hydrochloric acid standard solution drop concentration.

protein powderproteinuncertaintyKjeldahl nitrogen determination method

苏君梅、黄玲、黎俊威、林湛皓、谢小浩、刘春丽

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捷通(广州)检测技术服务有限公司,广东 广州 510730

蛋白粉 蛋白质 不确定度 凯氏定氮法

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
年,卷(期):2024.(31)