Objective:To establish a method for rapid determination of vitamin B1,vitamin B2 and vitamin B9 in food nutritional supplements by ultra performance liquid chromatography(UPLC).Method:The sample was dissolved in methanol solution of potassium dihydrogen phosphate,extracted by oscillation,and filtered by centrifugation,and its content was determined by liquid chromatograph with ultraviolet detector,and quantified by external standard method.Result:Vitamin B1,vitamin B2 and vitamin B9 had a good linear relationship in their respective concentration ranges,with linear correlation coefficients of 0.999 20,0.999 96 and 0.999 99,respectively;the average recoveries were 92.21%to 98.09%,94.11%to 100.29%and 97.55%to 101.74%,respectively.Conclusion:This method is rapid,simple and effective,and is suitable for the determination of vitamin B1,vitamin B2 and vitamin B9 in food supplements.
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