Objective:To establish a method for the determination of bacterial endotoxin in meat and meat products by enzyme-linked immunoassay.Method:According to the quantitative relationship between antibody and the amount of colored groups released by the adsorption reaction of endotoxin,the standard curve was drawn to calculate the content of bacterial endotoxin in meat and meat products,and the detection method of endotoxin in meat and meat products was established.Result:In the range of 0～60 EU·mL-1,the correlation coefficient was greater than 0.99,the recovery rate was 96.9%～103.9%,and the relative standard deviation was 3.30%.Conclusion:This method can meet the requirements of rapid detection of bacterial endotoxin in meat and meat products.
关键词
肉及肉制品/细菌内毒素/酶联免疫法
Key words
meat and meat products/bacterial endotoxin/enzyme-linked immunoassay
维普
万方数据