Determination of Deoxynivalenol in Coicis Semen by HPLC
Objective:To establish a method for the determination of deoxynivalenol in Coicis Semen by immunoaffinity column purification combined with high performance liquid chromatography.Method:Coicis Semen was crushed and extracted with water.After immunoaffinity column sampling,purification and elution,the content of deoxynivalenol was determined by HPLC.Result:In the range of 40～400 ng·mL-1,the concentration of deoxynivalenol showed a good linear relationship with the peak area,the correlation coefficient was 1,the detection limit was 9.6 μg·kg-1,the limit of quantification was 28.8 μg·kg-1,the recoveries were 90.7%～106.2%,and the relative standard deviations were less than 4%.Conclusion:The method is simple,specific and precise,and can be used for the determination of deoxynivalenol in Coicis Semen.
万方数据
维普
