Real Time qPCR Based on SYBR Green for Quantitative Detection of RNA in Animal Fat
This article establishes a method for extracting RNA from edible animal fats and using SYBR Green based real-time fluorescent quantitative PCR(RT-qPCR)to determine RNA expression levels in different animal fats.The results indicate that the improved extraction method TRIzol® can effectively extract high-purity RNA of 200 ng·μL-1 to 500 ng·μL-1 from animal fat.After RT qPCR reaction,all extracted RNA samples exhibited excellent amplification performance.The extraction method TRIzol® developed in this study,combined with RT-qPCR,can provide data support for the identification and tracing of animal fat in the future.
万方数据
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