Digital polymerase chain reaction(dPCR)is a new absolute quantitative analysis technology for nucleic acids,which can accurately detect the initial DNA copy number of samples by limiting dilution sample and Poisson probability distribution principle without requiring standard materials and drawing standard curves.It has the characteristics of strong specificity,high sensitivity and good repeatability,and has good development potential in the field of food safety nucleic acid detection.This paper introduces the basic principle and characteristics of dPCR technology,analyzes the research status of dPCR technology in the field of food safety nucleic acid detection,and prospects its application in food safety,in order to provide some references for promoting the application of dPCR in the field of food safety nucleic acid detection.
关键词
数字聚合酶链式反应(dPCR)/食品安全/核酸检测/绝对定量
Key words
digital polymerase chain reaction(dPCR)/food safety/nucleic acid detection/absolute quantification
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