Research Progress of Digital Polymerase Chain Reaction Technology in Food Safety Nucleic Acid Detection
Digital polymerase chain reaction(dPCR)is a new absolute quantitative analysis technology for nucleic acids,which can accurately detect the initial DNA copy number of samples by limiting dilution sample and Poisson probability distribution principle without requiring standard materials and drawing standard curves.It has the characteristics of strong specificity,high sensitivity and good repeatability,and has good development potential in the field of food safety nucleic acid detection.This paper introduces the basic principle and characteristics of dPCR technology,analyzes the research status of dPCR technology in the field of food safety nucleic acid detection,and prospects its application in food safety,in order to provide some references for promoting the application of dPCR in the field of food safety nucleic acid detection.
digital polymerase chain reaction(dPCR)food safetynucleic acid detectionabsolute quantification
万方数据
维普
