Establishment of UPLC Method for Determining the Content of Ellagic Acid and Rhoifolin in Drinks
Objective:To establish a ultra performance liquid chromatography(UPLC)method for simultaneous determination of ellagic acid and rhoifolin content in drink.Method:Waters Acquity BEH C18 liquid chromatography column(2.1 mm×50 mm,1.7 μm)was used,and the mobile phase was acetonitrile-0.1%formic acid aqueous solution(10∶90,V/V),the flow rate was 0.4 mL·min-1,the column temperature was 30℃,and the detection wavelength was 254 nm(ellagic acid)and 203 nm,respectively.Result:The linear relationships of ellagic acid and aracin were good in their respective concentration ranges,and the correlation coefficients were 0.998 5 and 0.999 5,respectively.The average recoveries were from 95.01%to 104.59%and from 96.49%to 104.51%,respectively,which met the requirements.Conclusion:The method is convenient,reliable and reproducible,and is suitable for the determination of ellagic acid and rhoifolin in drinks.
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