UPLC法测定饮品中鞣花酸和野漆树苷的含量
Establishment of UPLC Method for Determining the Content of Ellagic Acid and Rhoifolin in Drinks
颜方亮 1李玉珍1
作者信息
- 1. 捷通(广州)检测技术服务有限公司,广东 广州 510730
- 折叠
摘要
目的:建立超高效液相色谱法(Ultra Performance Liquid Chromatography,UPLC)同时测定饮品中鞣花酸和野漆树苷含量的方法.方法:采用Waters Acquity BEH C18 液相色谱柱(2.1 mm×50 mm,1.7 μm),流动相为乙腈-0.1%甲酸水溶液(10∶90,V/V),流速为0.4 mL·min-1,柱温为30℃,检测波长分别为254 nm(鞣花酸)和 203 nm(野漆树苷).结果:鞣花酸和野漆树苷在各自浓度范围内线性关系良好,相关系数分别为 0.998 5 和 0.999 5,平均加标回收率分别在 95.01%～104.59%和 96.49～104.51%,均符合要求.结论:该方法便捷、可靠、重复性好,适用于测定饮品中鞣花酸和野漆树苷的含量.
Abstract
Objective:To establish a ultra performance liquid chromatography(UPLC)method for simultaneous determination of ellagic acid and rhoifolin content in drink.Method:Waters Acquity BEH C18 liquid chromatography column(2.1 mm×50 mm,1.7 μm)was used,and the mobile phase was acetonitrile-0.1%formic acid aqueous solution(10∶90,V/V),the flow rate was 0.4 mL·min-1,the column temperature was 30℃,and the detection wavelength was 254 nm(ellagic acid)and 203 nm,respectively.Result:The linear relationships of ellagic acid and aracin were good in their respective concentration ranges,and the correlation coefficients were 0.998 5 and 0.999 5,respectively.The average recoveries were from 95.01%to 104.59%and from 96.49%to 104.51%,respectively,which met the requirements.Conclusion:The method is convenient,reliable and reproducible,and is suitable for the determination of ellagic acid and rhoifolin in drinks.
关键词
饮品/鞣花酸/野漆树苷/超高效液相色谱法(UPLC)Key words
drink/ellagic acid/rhoifolin/ultra performance liquid chromatography(UPLC)引用本文复制引用
出版年
2024
NETL
NSTL
万方数据