紫外-可见分光光度法测定饮料中柠檬黄的研究
Study on the Determination of Lemon Yellow in Beverages by UV Visible Spectrophotometry
王开辉1
作者信息
- 1. 深圳市计量质量检测研究院,广东 深圳 518000
- 折叠
摘要
采用银纳米颗粒与柠檬黄结合反应,建立了紫外-可见分光光度法测定饮料中柠檬黄的方法.通过单因素实验法筛选出最佳的测定条件为加入3.0 mL银纳米颗粒分散液做反应试剂,调节体系pH值为6.0,在 4℃下反应 20 min后于波长400 nm处测定吸光度.经过进一步方法学验证表明,该方法线性范围较宽、线性关系、准确度与精密度良好,可用于饮料中柠檬黄含量的检测.
Abstract
The method for determining lemon yellow in beverages using UV visible spectrophotometry was established by combining silver nanoparticles with lemon yellow.The optimal measurement conditions were selected through a single factor experiment method,which involved adding 3.0 mL of silver nanoparticle dispersion as the reaction reagent,adjusting the pH of the system to 6.0,reacting at 4℃for 20 minutes,and measuring the absorbance at a wavelength of 400 nm.Further methodological validation has shown that this method has a wide linear range,good linear relationship,and good accuracy and precision,and can be used for the detection of lemon yellow content in beverages.
关键词
分光光度法/饮料/柠檬黄Key words
spectrophotometry/drink/lemon yellow引用本文复制引用
出版年
2024
NETL
NSTL
万方数据