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紫外-可见分光光度法测定饮料中柠檬黄的研究

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采用银纳米颗粒与柠檬黄结合反应,建立了紫外-可见分光光度法测定饮料中柠檬黄的方法.通过单因素实验法筛选出最佳的测定条件为加入3.0 mL银纳米颗粒分散液做反应试剂,调节体系pH值为6.0,在 4℃下反应 20 min后于波长400 nm处测定吸光度.经过进一步方法学验证表明,该方法线性范围较宽、线性关系、准确度与精密度良好,可用于饮料中柠檬黄含量的检测.
Study on the Determination of Lemon Yellow in Beverages by UV Visible Spectrophotometry
The method for determining lemon yellow in beverages using UV visible spectrophotometry was established by combining silver nanoparticles with lemon yellow.The optimal measurement conditions were selected through a single factor experiment method,which involved adding 3.0 mL of silver nanoparticle dispersion as the reaction reagent,adjusting the pH of the system to 6.0,reacting at 4℃for 20 minutes,and measuring the absorbance at a wavelength of 400 nm.Further methodological validation has shown that this method has a wide linear range,good linear relationship,and good accuracy and precision,and can be used for the detection of lemon yellow content in beverages.

spectrophotometrydrinklemon yellow

王开辉

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深圳市计量质量检测研究院,广东 深圳 518000

分光光度法 饮料 柠檬黄

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
年,卷(期):2024.(33)