基于液相色谱-质谱/质谱联用技术的兽药残留检测方法优化研究
Optimization of Veterinary Drug Residue Detection Method Based on Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
林扬1
作者信息
- 1. 深圳中检联检测有限公司,广东 深圳 518000
- 折叠
摘要
本文采用液相色谱-质谱/质谱联用技术检测水产品中的磺胺类兽药残留.结果表明,在 2～800 ng·mL-1 的线性范围下,5 种磺胺类兽药的相关系数均大于 0.99,说明方程的线性关系良好;5 种磺胺类兽药的方法检出限为 0.002～0.120 μg·kg-1,定量限为 0.012～0.370 μg·kg-1;在 25 μg·kg-1、50 μg·kg-1、100 μg·kg-1 的加标水平下,5 种磺胺类兽药在水产品中的平均回收率为58.10%～124.37%,相对标准偏差为5.63%～15.03%.
Abstract
This article uses liquid chromatography-mass spectrometry/mass spectrometry to detect residues of sulfonamide veterinary drugs in aquatic products.The results showed that within the linear range from 2 ng·mL-1 to 800 ng·mL-1,the correlation coefficients of the five sulfonamide veterinary drugs were all greater than 0.99,indicating a good linear relationship of the equation;the detection limit of five sulfonamide veterinary drugs rang from 0.002 μg·kg-1 to 0.120 μ g·kg-1,and the quantification limit range from 0.012 μg·kg-1 to 0.370 μg·kg-1;at spiked levels of 25 μg·kg-1,50 μg·kg-1,and 100 μg·kg-1,the average recovery rates of five sulfonamide veterinary drugs in aquatic products was 58.10%to 124.37%,with a relative standard deviation of 5.63%to 15.03%.
关键词
兽药残留检测/液相色谱/质谱联用技术/流动相参数Key words
veterinary drug residue detection/liquid chromatography/mass spectrometry combined technology/mobile phase parameters引用本文复制引用
出版年
2024
NETL
NSTL
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