高效液相色谱法测定食品中的纳他霉素
Determination of Natamycin in Food by High Performance Liquid Chromatography
吴金松 1周泽崧 1陈如波 1陈浩朗1
作者信息
- 1. 广东省绿色产品认证检测中心有限公司,广东 广州 510440
- 折叠
摘要
目的:建立一种简单快速的样品前处理方法结合高效液相色谱法测定糕点、苹果汁和卤猪肉中的纳他霉素.方法:样品经75%甲醇水溶液、超声波提取,经色谱柱分离,以甲醇-0.1%甲酸水溶液为流动相进行等度洗脱,利用高效液相色谱仪进行定性和定量分析.结果:纳他霉素在0.1～10.0 μg·mL-1 的浓度范围内线性关系良好,相关系数大于0.995,检出限为0.1 mg·kg-1.对糕点、苹果汁和卤猪肉进行加标回收实验,平均回收率为 83.24%～101.60%,相对标准偏差为1.51%～8.21%(n=6).结论:本方法操作简便快速、灵敏度高,准确性和精密度良好,可满足食品中纳他霉素快速筛查和定量分析的需求.
Abstract
Objective:To establish a simple and rapid sample pretreatment method combined with high performance liquid chromatography(HPLC)for the determination of natamycin in pastry,apple juice and brine pork.Method:The samples are extracted by ultrasonication with 75%methanol aqueous solution,separated by chromatography column,and eluted by gradient washing with a mobile phase of methanol-0.1%formic acid aqueous solution,and analyzed qualitatively and quantitatively by HPLC.Result:The linearity of natamycin in the concentration range of 0.1～10.0 μg·mL-1 is good,the correlation coefficient is greater than 0.995,and the detection limit is 0.1 mg·kg-1.The average recovery of pastry,apple juice and brine pork is from 83.24%to 101.60%,and the relative standard deviation is from 1.51%to 8.21%(n=6).Conclusion:The method is simple,rapid,sensitive,accurate and precise,and can meet the needs of rapid screening and quantitative analysis of natamycin in food.
关键词
纳他霉素/高效液相色谱法/糕点/苹果汁/卤猪肉Key words
natamycin/high performance liquid chromatography/pastry/apple juice/brine pork引用本文复制引用
出版年
2024
NETL
NSTL
万方数据