Determination of Natamycin in Food by High Performance Liquid Chromatography
Objective:To establish a simple and rapid sample pretreatment method combined with high performance liquid chromatography(HPLC)for the determination of natamycin in pastry,apple juice and brine pork.Method:The samples are extracted by ultrasonication with 75%methanol aqueous solution,separated by chromatography column,and eluted by gradient washing with a mobile phase of methanol-0.1%formic acid aqueous solution,and analyzed qualitatively and quantitatively by HPLC.Result:The linearity of natamycin in the concentration range of 0.1～10.0 μg·mL-1 is good,the correlation coefficient is greater than 0.995,and the detection limit is 0.1 mg·kg-1.The average recovery of pastry,apple juice and brine pork is from 83.24%to 101.60%,and the relative standard deviation is from 1.51%to 8.21%(n=6).Conclusion:The method is simple,rapid,sensitive,accurate and precise,and can meet the needs of rapid screening and quantitative analysis of natamycin in food.
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