Study on the Detection Method and Elimination Pattern of Residual Eugenol in Grouper
Objective:To establish a method for the determination of eugenol residues in grouper by gas chromatography-tandem triple quadrupole mass spectrometry and to study the digestion law of eugenol in grouper.Method:The samples were extracted with ethyl acetate and separated on a DB-5 capillary column.The samples were quantified by external standard method in multiple reaction monitoring mode.Result:Eugenol had good linear relationship in the concentration range of 10～200 ng·mL-1,with a linear coefficient greater than 0.999 0.The limits of detection and quantification were 5 µg·kg-1 and 10 µg·kg-1,respectively.When the concentration was 10～100 µg·kg-1,the average spike recovery was 99.3%～111.4%,and the relative standard deviation was 1.12%～7.54%.Conclusion:This method has simple pretreatment operation,high sensitivity and good reproducibility,and is suitable for the detection of eugenol residues in grouper.Eugenol was eliminated rapidly in grouper within the first 3 days,and the elimination rate tended to be slow over time.
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