Detection of Aflatoxin B1 Content in Corn and Its Products by High-Performance Liquid Chromatography
A method for detecting aflatoxin B1 content in corn and its products using high-performance liquid chromatography has been established in this paper.The sample was extracted by vortex oscillation with methanol water solution,and the extract was purified by an immunoaffinity column and concentrated by nitrogen blowing for determination by high-performance liquid chromatography fluorescence detector.The results showed that aflatoxin B1 had a good linear relationship in the range of 0.1～20.0 ng·mL-1,with a detection limit of 0.02 μg·kg-1 and a quantification limit of 0.05 μg·kg-1.The recovery rate was within the range of 89.0%～98.7%,and the relative standard deviation was 1.24%～4.39%.This method is applicable for the detection of aflatoxin B1 content in corn and its products.
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