Determination of β-Carotenoids in Solid Beverages and Confectionery by High Performance Liquid Chromatography
Objective:To establish an extraction method for β-carotene in solid beverages and candies,and to determine the content of β-carotene in the samples using high-performance liquid chromatography.Method:Weigh a uniform sample,add antioxidant,disperse and dissolve in water,extract with petroleum ether acetone(1∶1),wash with water,concentrate,and then dissolve again.Determine the content of β-carotene by HPLC.Result:The linear coefficient R was 0.999 5 within the range of β-carotene content from 0.5 μg·mL-1 to 10.0 μ g·mL-1,showing good linearity.The sample recovery rate was from 91.9%to 99.3%,and the average relative standard deviation was from 1.14%to 3.59%.Conclusion:This method is simpler and faster compared to GB 5009.83-2016,and can effectively reduce oxidative losses during pretreatment.It can be used for the determination of β-carotene in solid beverages and candies.
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