Study on Antioxidative Effect and Mechanism of Chlorogenic Acid
Objective:To study the effect of improvement and related mechanism of chlorogenic acid on oxidation-damaged cells.Method:Human embryonic kidney 293 cells(HEK293 cells)cultured in vitro were induced by 2,2'-azobis-2-methyl-prolimidamide(AAPH)to establish the oxidative damage model,and chlorogenic acid was given to intervention.Antioxidant indexes such as glutathione peroxidase activity,malondialdehyde content,superoxide dismutase activity and the expression of oxidative damage related pathway proteins in the cells were determined.Result:Reactive oxygen species levels and malondialdehyde content were reduced by chlorogenic acid(p＜0.05).Glutathione content,glutathione peroxidase activity,catalase activity and superoxide dismutase activity were incerased by chlorogenic acid(p＜0.05).And chlorogenic acid can improve cellular oxidative damage by regulating the Kelch-like ECH-associated protein 1/heme oxygenase 1(Keap1/HO-1)pathway and reducing the expression of NADPH oxidase 4(NOX4)(p＜0.05).Conclusion:The antioxidant mechanism of chlorogenic acid may be related to its regulation of the Keap1/HO-1 pathway and NOX4 protein.
chlorogenic acidantioxidantwestern blotKelch-like ECH-associated protein 1/heme oxygenase 1(Keap1/HO-1)pathwayNADPH oxidase 4(NOX4)
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