In this paper,the extraction technology of total flavonoids from fresh fruit of Lycium barbarum L.is optimized by single factor test and orthogonal test,and the flavonoid components of fresh fruit of Lycium barbarum L.are determined by high performance liquid chromatography.The results show that the optimal extraction conditions are ultrasonic extraction,solid-liquid ratio of 1∶10(g∶mL),ultrasonic frequency of 50 Hz and extraction time of 30 min.The precision,stability and reproducibility of high performance liquid chromatography meet the requirements of methodology and the recovery rate is good,so it can be used as a method for the detection of flavonoids in fresh fruits of Lycium barbarum L..Rutin is the main component of flavonoids in fresh fruits of Lycium barbarum L.in Ningxia.The contents of total flavonoids and rutin in Ningqi No.5 are higher than those in the other three varieties,and the contents of total flavonoids and rutin in Ningqi No.2 are the lowest.
关键词
枸杞鲜果/总黄酮/提取/组成
Key words
fruits of Lycium barbarum L./total flavonoids/extraction/composition
维普
万方数据