Detection of Diazepam Residues in Aquatic Products by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
An ultra-high performance liquid chromatography-tandem mass spectrometry method for the determination of diazepam residues in aquatic products was developed.The sample was extracted by acetonitrile,purified by a combination of enhanced lipid removal agent EMR-Lipid and Cleanert LipoNo,and quantified by internal standard method using positive ion multi-reaction monitoring mode.The results showed that the linear relationship was good when the concentration of diazepam was 1 ng·L-1 to 50 ng·L-1,R2=0.999 2;the limits of detection and quantification were 0.23 μg·kg-1 and 0.43 μg kg-1,respectively;the matrix effect of diazepam on perch,grass carp,silver carp and crucian fish was 0.85 to 1.15,and the matrix effect was not obvious;the average recoveries of samples were 87.3％to 120.2％,and the relative standard deviations were 3.27％to 11.02％.The method can achieve rapid quantitative detection of diazepam in aquatic products,and provide technical support for quantitative analysis of veterinary drug residues in aquatic products.
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维普
