Detection of Yersinia enterocolitica in Meat Product by Real-Time PCR
Objective:To further enhance the detection efficiency of Yersinia enterocolitica in meat products.Method:Sample processing and bacteria enriching were according to the national food safety standard GB 4789.8-2016,and the bacteria enrichment broth was firstly detected by real-time polymerase chain reaction,then Yersinia enterocolitica strains were isolated from the positive samples and screened by real-time PCR again,finally the positive strains were confirmed by biochemical identification.Result:Real-time PCR method can detect Yersinia enterocolitica with low contamination level(1～10 CFU·mL-1)in meat products.34 Yersinia enterocolitica positive samples were detected from 352 meat products,and the total detection rate was 9.66％,the detection rate of poultry meat was 8.94％(27/302)and 14.0％(7/50)for pork.Conclusion:There was relatively high contamination rate of Yersinia enterocolitica in meat products.The method of real-time PCR combined with biochemical identification can improve the detection efficiency and reduce missed detection.
万方数据
维普
