Characterization of key odor compounds in steamed Chinese mitten crab (Eriocheir sinensis) farmed in Chongming region by monolithic material sorptive extraction-gas chromatography-olfatometry and odor activity value methods
Objective To analyze the volatile compounds in different edible parts of steamed male E. si-nensis farmed in Chongming region, select the key odor compounds (KOCs) from all the volatiles, and finally offer some theoretical basis to explain its outstanding flavor. Methods Seven adsorption rods made of Mono-Trap RCC18 were applied to extract the volatile compounds of 5 g crab sample under 100℃for 50 min under a headspace extraction mode. Those volatiles of crab samples were then separated and identified by GC-MS and quantified by an internal standard method. In order to select the KOCs from all the volatiles, a combination of GC-O and OAV methods were also employed. Results A total of 71 volatiles were finally identified in male E. sinensis farmed in Chongming region, among which 50, 48, 52 and 60 volatiles were identified in the abdomen, claw, leg meat and the gonad part, respectively. 19 KOCs were successfully selected from all 71 volatiles. Among them, 6 main key odor compounds (MKOCs), with an odor intensity value (OIV) over three and an odor activity value (OAV) over ten, were eventually obtained. Trimethylamine (fishy) was the only MKOC in all four parts. Besides, benzaldehyde (almond-like) and (Z)-4-heptenal (fishy) were the MKOC in the abdomen meat and leg meat, respectively. 3 MKOCs were found in the gonad part, including hexanal (grassy), 1-octen-3-ol (mushroom) and nonanal (grassy). Conclusion Six MKOCs were verified to have relative high odor activities by both OAV and GC-O methods. Those MKOCs might represent the key odor characteristics of different edible parts of male E. sinensis farmed in Chongming region. However, the task of revealing forma-tion mechanism for these MKOCs remains to be carried out in the future.
Eriocheir sinensisMono Trapgas chromatography-olfactometryodor intensity valueodor activity value
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NSTL
万方数据
维普
