Detection of volatile components in rapeseed by solid phase micro-extraction-gas chromatography-mass spectrometry
Objective The volatile components of normal rapeseed and mildew rapeseed have been identified and classified by gas chromatography-mass spectrometry (GC-MS) technology. Methods The headspace solid phase microextraction (HS-SPME) technology has been used to extract volatile components in rapeseed, and the headspace solid phase microextraction head, extraction temperature, extraction time and the analytic conditions were optimized. Finally, gas chromatography-mass spectrometry (GC-MS) technology was used for the determination of volatile components in rapeseed and mildew rapeseed. Results The optimized conditions of HS-SPME were extraction fiber of 30/50μm DVB/CAR/PDMS, extraction temperature of 60℃, extraction time of 60 min, and desorption time of 3 min, respectively. Under the optimal experimental conditions, the original samples and the mildew samples could be distinguished by SPME-GC-MS. The results showed that volatile compounds in rapeseed were aldehydes, alcohols, ketones, hydrocarbons, some acid ester compounds, and heterocycle group, in which alkane was the most group. 3-symplectic ketone, acetophenone, benzene acetaldehyde, phenylethyl alcohol, and alkane compounds such as nonadecane and heneicosane were only detected in the mildew rapeseed. Conclusion This method can better distinguish the normal rapeseed and mildew rapeseed.
NETL
NSTL
万方数据
维普
