两种检测SOD酶活性方法的比较
The comparison of two methods of testing superoxide dismutase activity
曲敏 1秦丽楠 1刘羽佳 1范宏臣 1朱姝 1王金凤1
作者信息
- 1. 哈尔滨商业大学食品科学与工程省级重点实验室，哈尔滨 150076
- 折叠
摘要
目的：比较邻苯三酚自氧化法和氮蓝四唑(NBT)光还原法分别测定超氧化物歧化酶(SOD)酶活力的差异。方法采用两种方法分别测定SOD标准品、苜蓿和番茄两种植物提取物的不同来源SOD酶活性,比较其精确性和灵敏度。结果在标准品SOD酶活的测定中,邻苯三酚自氧化法和NBT光还原法测酶活性的变异系数分别为0.84、2.03,而进样量后者是前者的2.6倍,稀释倍数是前者的6倍,邻苯三酚自氧化法的精确度高于NBT 光还原法,但灵敏度远不及后者。在测定两种植物 SOD 时,邻苯三酚自氧化法测得酶活性变异系数是NBT光还原反应测得酶活性变异系数的1.30~1.47倍, NBT光还原法具有稳定性、重现性好的特点,优于邻苯三酚自氧化法。结论 NBT光还原法是测定植物SOD及SOD标准品酶活的理想检测方法。
Abstract
ObjectiveTo compare the pyrogallol autoxidation and nitroblue tetrazolium (NBT) photore-duction methods for determination of superoxide dismutase (SOD) enzyme activity.MethodsThe paper based on the different methods to determine the SOD activity of standard, alfalfa and tomato, respectively, also compare the accuracy and sensitivity. ResultsThe results showed that pyrogallol autoxidation and NBT pho-toreduction method of enzyme activity coefficient of variation were 0.84, 2.03, and the sample quantity which was 2.6 times of the former, dilution ratio was 6 times of the former, and the accuracy of pyrogallol autoxida-tion was higher than NBT photoreduction method but the sensitivity was poor, in detecting the SOD activity of standard. However, Pyrogallol autoxidation activity coefficient of variation was measured by NBT photoreduc-tion reaction between 1.30 to 1.47 times the activities was measured by the coefficient of variation, the NBT photoreduction method has preferable stability and reproducibility than pyrogallol autoxidation in determining the SOD activity.ConclusionNBT photoreduction is an ideal testing method to determine the SOD activity of standard and plants.
关键词
超氧化物歧化酶/酶活力/邻苯三酚自氧化法/氮蓝四唑光还原法Key words
superoxide dismutase/pyrogallol autoxidation/NBT photoreduction method引用本文复制引用
基金项目
黑龙江省自然科学基金(C2011-24)
黑龙江省教育厅科学技术研究项目(12511128)
黑龙江省博士后科研启动基金(LBH-Q13098)
出版年
2014
NETL
NSTL
维普
万方数据