Objective To compare the preconditioning efficiencies of ethyl carbamate in wine by liquid-liquid extraction (LLE), solid phase extraction (SPE) and dispersive solid phase extraction (d-SPE). MethodThree methods of LLE with dichloromethane, SPE with Cleanert EC column and d-SPE with acetonitrile, graphitized carbon and primary secondary amine were separately used to extract the ethyl carbamate of the wine samples, which was analyzed by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring mode.Result The spiked recoveries at 3 levels of these methods were in the range of 70%~110% and the relative standard deviations (n=5) were less than 6%, indicating that those methods had a good accuracy and precision. Since it was low-cost and had a high enrichment efficiency, LLE was suitable for pretreatment of samples with trace amount and could meet the requirements of compliance detection in import and export manufacturers. SPE was more appropriate for high through-put determination due to convenience and fast speed. d-SPE could be well applied to various hazard substances screening, because it was mid-cost, best recovery and high comprehensive efficiency.Conclusion In consideration of the pretreatment efficiency and cost, d-SPE was more suitable for the daily quality control inspection in wine manufacturers. d-SPE coupled with GC-MS had been applied to determination of ethyl carbamate in wine.
NETL
NSTL
万方数据
维普
