Technology optimization for extracting proanthocyanidin from spring rapeseed hull in Qinghai province
Objective To increase the yield of extracting proanthocyanidin from rapeseed skin, the methods of extraction were studied, and the technology of extraction was optimized. Methods The single factor tests of ethanol volume fraction, extraction temperature and extraction time were studied to explore the effect on extraction of proanthocyanidin in rapeseed skin. The optimum extraction processing was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. Results The optimum extraction conditions were that ethanol volume fraction was 63.7%, extraction temperature was 58.9 ℃ and extraction time was 53 min. Under these optimized conditions, the experimental value of proanthocyanidin extraction yield was 5.16 mg/g, which was exactly in accordance to the predicted value 5.22 mg/g. The proanthocyanidin extracted process of spring rapeseed skin was optimized by response surface methodology, while validate test results were close to theoretical values under the optimum extraction conditions. Conclusion The extraction proanthocyanidin from rapeseed hull using response surface methodology is feasible and reliable.
NETL
NSTL
万方数据
维普
