Optimization of extraction technology and determination of flavonoid in Huize radix puerariae
Objective To optimize the extraction of flavonoid in Huize radix puerariae and measure its content.Methods The extraction of crude flavonoid from Huize radix puerariae was optimized by L9(34) orthogonal experiments,which was purified by the macro-porous resin adsorption and then the content of flavonoid was determined by the ultraviolet spectrophotometry.Results The optimum extraction method was the alkaline extraction method,the lye concentration was 4％,the solid-liquid ratio was 1∶15(m/V),the power of micro-wave oven was 350 W,the extraction time was 4 min and the extraction times was 3 times.With rutin as standard solution,the flavonoid content was 7.8617％ at 357 nm.Conclusion This method may be a novel method for the extraction and determination of the flavonoid in Huize radix puerariae.
huize radix puerariaeflavonoidalkaline extraction methodoptimization process
NETL
NSTL
万方数据
维普
