Objective To optimize the extraction method of eicosapentaenoic acid microalgae oil by solvent extraction with ultrasonic assistance,with Nannochloropsis sp.riched in EPA as raw materials,and analyze the composition of fatty acid of the extracted microalgae oil.Methods Samples were added at a certain ratio of liquid extraction solvent,and extracted for a certain time with ultrasonic assistance after thorough mixing.Then the mixed samples were centrifuged at speed of 3000 r/min with 5 min.At last,the supematant were dried via rotary evaporation method and then were dried at 105 ℃.The extracted microalgae oil's fatty acid compositions were analyzed by gas chromatography after methyl esterification.Results The optimal extraction conditions were as follows:temperature 50 ℃,extraction time 30 min,liquid-solid ratio 15∶1,3 times of extraction.The extraction rate of microalgae oil reached 37.6％ under the optimal extraction conditions.Gas chromatography analysis showed that EPA,the main component of the microalgae oil was 39.0％ in total fatty acids.Conclusion The proposed method is simple,and can get high extraction rate of microalgae oil and high content of EPA,which is suitable for extracting microalgae oil from Nannochloropsis sp.The technology will provide a scientific basis for industrialization of microalgae oil of EPA.
NETL
NSTL
万方数据
维普
