Identification of adulterated honey by precolumn derivatization-high performance liquid chromatography-tandem mass spectrometry
Objective To establisha method for the determination of adulterated honey.Methods Honey samples were dissolved and treated by vacuum drying, and then they were fluorescent labeled before separated by high performance liquid chromatography (HPLC) using NH2 column with acetonitrile and ammonium formate as mobile phase, and qualitatively analyzed by ESI-MS under SIR mode.Results It was shown that the fluorescent labeling of oligosaccharides with reducing end in honey were effectively achieved when using 2-aminobenzamide and 2-picoline-borane following by reductive amination reaction. It was also shown that natural honey contained monosaccharide (DP1), disaccharide (DP2) and trisaccharide (DP3); in addition, adulterated honey contained tetrasaccharide (DP4), pentasaccharide (DP5) and hexasaccharide (DP6).Conclusion The results of the established method are basically consistent with those of the national standard method, and the established method do not need to purify the samples before HPLC analysis, which provide references for the rapid screening method of adulterated honey.
adulterated honeyoligosaccharides with reducing endprecolumn derivatizationhigh performance liquid chromatography-tandem mass spectrometry
NETL
NSTL
万方数据
维普
