Antioxidative and cholesterol removal ability of Enterococcus mundtii Sna
Objective To get a good strain of lactic acid bacteria Sna isolated from pheasant crop and investigate the effects of free radical-scavenging and cholesterol-reducing. Methods Strain Sna was identified by colony morphology, cell morphology, biochemical test and 16S rRNA sequence analysis, while antioxidant ability and cholesterol lowering effects were completed in vitro. Results Strain Sna belonged to Enterococcus mundtii. Under cell concentration of 109 CFU/mL, the radical-scavenging ratios on hydroxyl free radical, DPPH, and superoxide anion free radical were 1.58%, 36.6%, and 57.1%, respectively. Cholesterol removal was greatly associated with growth of cultures, with the passage of time reaching its maximum (5.14 μg/mg of dry weight) in 36 h. The cholesterol assimilation by growing cells was 4.96 μg/mg of dry weight, which was significantly higher than that of the resting and dead cells. While cholesterol removed by dead and resting cells were 4.39 μg/mg and 4.23 μg/mg of dry weight, respectively. Conclusion Strain Sna shows free radical-scavenging ability and the cholesterol-reducing ability. These evidences indicate cholesterol removal by adsorption and absorption in the process of growth. Strain Sna could be applied to functional food formulations, which could resist oxidation and lower blood cholesterol levels.
NETL
NSTL
万方数据
维普
