Taqman fluorescent PCR method for rapid identification of Ruditapes philippinarumus and its products
Objective To establish a method of qualitative detection of Ruditapes philippinarumus by Taqman fluorescence PCR. Methods A pair of Ruditapes philippinarumus specific primers were designed according to conservative gene sequence in the CoxI gene of the mitochondrial cytochrome oxidase of Ruditapes philippinarumus. The PCR amplification reaction was carried out by Taqman fluorescence PCR method, and the specificity of the Ruditapes philippinarumus was tested. Results The specificity was good between primers and Ruditapes philippinarumus DNA. Ruditapes philippinarumus DNA detection limit was 0.04 ng/μL, which could reach 0.001%of the mass fraction of Ruditapes philippinarumus meat powder. Conclusion This method is highly specific and sensitive, which can be used for the identification of the authenticity of Ruditapes philippinarumus in the food.
NETL
NSTL
万方数据
维普
