Determination of 15 kinds of chemicals illegally added in prefabricated food by ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry
Objective To establish a method for the determination of 15 kinds of chemicals illegally added in prefabricated food by ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry in multiple reaction monitoring-information dependent acquisition-enhanced product ion(MRM-IDA-EPI)mode combined with pass-through solid phase extraction technology.Methods The samples were extracted with 1%(volume fraction)formic acid in acetonitrile.The extractive solution was purified by pass-through solid phase extraction column Captiva EMR-Lipid,and the purified liquid was separated on a Agilent Poroshell 120 EC-C18(2.1 mm×100 mm,1.9 μm)column.The mobile phase was acetonitrile and 5 mmol/L aqueous ammonium acetate containing 0.05%formic acid.The target compounds were detected by MRM-IDA-EPI mode with external standard method.Results The calibration curves of the 15 kinds of compounds were linear in the concentration range within limits with correlation coefficients between 0.9956 and 0.9996.The limits of detection ranged from 0.62-62.50 μg/kg.The average recoveries of 15 kinds of compounds at 3 different levels ranged from 78.33%-109.51%with accuracy of 0.67%-14.02%.Conclusion The method can be applied to the determination of 15 kinds of chemicals illegally added in prefabricated food with its rapidity,high accuracy and high precision,providing support for quality control and market regulation of prefabricated food.
illegal additivesprefabricated foodpass-through solid phase extractionlinear ion trap mass spectrometrymultiple reaction monitoring-information dependent acquisition-enhanced product ion
万方数据
维普
