Efficient removal of cholesterol from fish oil by methyl-β-cyclodextrin
Objective To study the effects of methyl-β-cyclodextrin(M-β-CD)on removing cholesterol from fish oil.Methods Cholesterol removal rate and fish oil recovery rate were used as key indicators to screen suitable materials for removing cholesterol from fish oil from β-cyclodextrin(β-CD)and its derivatives.The optimal cholesterol removal process conditions were determined through orthogonal experiments,and the quality of fish oil before and after cholesterol removal was compared.Results M-β-CD was the best material for removing cholesterol from fish oil.The optimal process conditions of M-β-CD removal of cholesterol in fish oil were as follows:M-β-CD dosage of 30%,temperature of 50℃,time of 15 min.The cholesterol removal rate was 51.34%,and the fish oil recovery rate was 84.84%under these conditions.When cholesterol was removed under the optimal process conditions,the acid value,peroxide value,and anisidine value of fish oil significantly decreased(P<0.05),while the iodine value did not change significantly(P>0.05).The content of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)significantly increased(P<0.05),the proportions of saturated fatty acids(SFA),monounsaturated fatty acids(MUFA),and polyunsaturated fatty acids(PUFA)were 31.33%,16.81%,and 51.86%of the total lipid content,respectively,with no significant changes observed among them(P>0.05).Conclusion The treatment of fish oil with M-β-CD not only efficiently removes cholesterol but also enhances the quality,making it a promising method for cholesterol removal from fish oil.
fish oilqualitymethyl-β-cyclodextrincholesterol removal ratefish oil recovery rate
万方数据
维普
