Study on the inhibition of melanogenesis and antioxidant damage of tuna skin collagen oligo-peptide
Objective To investigate the inhibitory effects of tuna skin collagen oligo-peptide(TSCP)on melanin production and oxidative damage protection in Hacat cells.Methods Through investigated the effects of TSCP on melanin and tyrosinase production in B16-f10 cells,analyzed the antioxidant activity in B16-f10 cells,and assessed its protective effect against hydrogen peroxide(H2O2)induced oxidative damage in Hacat cells,the inhibitory mechanism of TSCP on melanin production and its protective mechanism against oxidative damage in Hacat cells were revealed.Results The experimental results showed that TSCP exhibits no significant cytotoxicity to B16-f10 cells at concentrations ranging from 0.01 to 1.00 mg/mL,and significantly inhibited tyrosinase activity and melanin production.At a concentration of 1.00 mg/mL,TSCP significantly reduced melanin content to 83.79%±4.31%(P<0.01)and tyrosinase activity to 78.14%±6.95%(P<0.001).TSCP also significantly reduced the levels of reactive oxygen species(ROS)in B16-f10 cells.At 1.00 mg/mL,the ROS levels decreased to 53.5%±4.4%of the control group(P<0.001),indicating potent antioxidant potential.Additionally,TSCP increased the activity of superoxide dismutase(SOD)to(16.62±0.62)U/mg prot(P<0.001)and reduced malondialdehyde(MDA)content to(0.352±0.051)U/mg prot(P<0.001).Glutathione peroxidase(GSH-Px)levels were also elevated,reaching(284.55±4.99)ng/mL at 1.0 mg/mL(P<0.01).In the Hacat cell model,TSCP similarly exhibited no significant toxicity and,in the H2O2-induced oxidative damage model,significantly improved cell viability and suppressed apoptosis.At 1.00 mg/mL,TSCP improved cell survival to 73.66%±5.48%(P<0.01),compared to the model group.Conclusion TSCP can inhibit melanin production,reduce oxidative stress,providing a foundation for its potential applications in skin whitening and antioxidant therapies.
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