Comparative study on 3 kinds of standard detection methods of Cronobacter in food
Objective To compare three domestic and foreign Cronobacter detection methods,for verifying the actual detection performance of three standard methods:GB 4789.40-2016 National standard for food safety food-Microbiology tests Cronobacter spp.(Enterobacter sakazakii)test,GB 4789.40-2024 National standard for food safety food-Microbiology tests Cronobacter test and ISO 22964-2017 Food chain microbiology-Horizontal detection methods for Cronobacter spp.Methods Three standard methods were used to detect Cronobacter in 403 samples collected from 2020 to 2022,including infant formula,cereal-based infant supplements,corn ingredients,and raw and supplemental ingredients of infant formula,and to test Cronobacter isolates for drug susceptibility.Results The total contamination rate of Cronobacter in 403 samples was 17.9%(72/403),and the contamination rates of corn raw material,cereal-based infant supplement and infant formula were 37.3%(38/102),23.4%(33/141)and 1.0%(1/100),respectively.Although there was no significant difference in the total detection rate among the three standard methods(χ2=3.601,P=0.170),41.5℃could effectively improve the detection rate compared with 44℃(χ2=18.813,P=0.000).The resistance rate of 72 strains of Cronobacter was 55.6%(40/72),among which the resistance rates of cefazolin,ampicillin,and amoxicillin/clavulanic acid were 52.8%(38/72),5.6%(4/72),and 1.4%(1/72),respectively.Conclusion This study shows that GB 4789.40-2024 has reached the level of the international relevant methods,providing data support for the mutual recognition of the test results of GB 4789.40 and ISO 22964 methods.Cronobacter has high resistance to cefazolin,which should be avoided when choosing antibiotics for clinical treatment.
CronobacterGB 4789.40ISO 22964comparison of methodantibiotic susceptibility
万方数据
维普
