Application of next-generation sequencing technology on microbial flora of fermented vegetables
Fermented vegetables are considered one of the most nutritious and beneficial foods for health.The research in the microbial community structure is a great significance to explore the dominant strain resources and improve the flavor quality of fermented vegetables.This paper summarized the application of the next-generation sequencing technology on microbial community of fermented vegetables.Some analysis methods were emphatically introduced involving with microbial community succession,Alpha diversity analysis,Beta diversity analysis,functional annotation(Kyoto Encyclopedia of Genes and Genomes metabolic pathway annotation,cluster of orthologous group annotation,gene ontology annotation),network and correlation analysis of environmental factors.Based on the research progress,this paper suggests implement combined application among the next-generation sequencing technology and multiple omics technology including transcriptomics,proteomics,metabolomics.The correlation between microbial function with color,flavor and texture of fermented vegetables should be investigated.The new strain resources with fermenting high-quality vegetables should be screened and obtained.It provides a theoretical basis for the transformation of traditional fermented vegetables to standardization,industrialization and modernization in China.