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红茶菌产细菌纤维素菌种分离与初步鉴定

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传统红茶菌在绿茶-蔗糖培养基表面会形成一层白色菌膜,取附着于菌膜上的菌体,在醋酸菌和酵母菌平板培养基上划线分离得到纯菌种A和Y,经菌落、菌体形态观察和生理生化试验鉴定,确定A为葡糖杆菌Gluconobacter asai和Y为路德类酵母Saccharomycodes ludwigli,进一步将两种菌株混合接入绿茶-蔗糖培养液中,对产物判定结果为纤维素。
Isolation and Identification of Microbes from Kombucha Producing Bacterial Cellulose
Traditional kombucha will form a layer of white membrane in green tea-sucrose medium surface. Microbes attached to the membrane were taken. The A and Y strains were isolated by crossing on the acetic acid bacterial and yeast plate medium. The two strains were isolated and identified Gluconobacter asai and Schizosaccharomyces pombeLinder by observing the colonies & cell morphology and physiological & biochemical tests separately. The mixture of the two strains was inoculated in green tea-sucrose medium and the products were identified as bacterial cellulose.

kombuchabacterial celluloseisolation & identificationacetic acid bacteriayeast

张妍、徐伟、傅徐阳

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哈尔滨商业大学食品工程学院,哈尔滨150076

国家乳制品质量监督检验中心,哈尔滨150086

红茶菌 细菌纤维素 分离鉴定 醋酸菌 酵母菌

2012

食品工业
上海市食品工业研究所

食品工业

北大核心
影响因子:0.47
ISSN:1004-471X
年,卷(期):2012.(10)
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