Experimental Study on Supercritical CO2 Extraction of Volatile Oil from Dipsacus asper
The volatile oil of Dipsacus asper was extracted by supercritical CO2 extraction,and the process condition was optimized by orthogonal test.The volatile oil was analyzed by gas chromatography-mass spectrometry(GC-MS),and the antioxidant properties of the volatile oil of Dipsacus asper in vitro were studied.The results showed that the optimum extraction condition of volatile oil from Dipsacus asper was as follows:anhydrous ethanol as the carrying agent,the ratio of material to the carrying agent 1∶2.5(g/mL),the extraction pressure 24 MPa,the extraction temperature 40 ℃,the extraction time 2.5 h,and the soaking time 1.0 h.The highest yield of volatile oil was 3.60％.The volatile oil of Dipsacus asperoides was analyzed by GC-MS.The main components were that palmitic acid(8.93％),ethyl palmitate(4.22％),ethyl linoleate(3.91％),ethyl linolenic acid(3.13％),octyl palmitate(2.54％)and β-sitosterol(10.51％).The antioxidant activity test showed that the inhibition rate of DPPH·was 83.4％±3.38％at the concentration of 16.00 mg/mL,and the inhibition rate of ABTS+·was 100％±0.10％at the concentration of 4.00 mg/mL,indicating that the extracted volatile oil had strong antioxidant activity.
supercritical CO2 extractionDipsacus aspervolatile oilsorthogonal testextraction technicsin-vitro antioxidant properties
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