Optimization of Enzymatic Hydrolysis of Bovine Bone Polypeptide and Its Antioxidant Activity Study
The enzymatic hydrolysis process of bovine bone peptides was optimized by response surface methodology.Three kinds of graded peptides with different molecular weights were obtained by preliminary separation of bovine bone peptides with different pore sizes,and their antioxidant activities in vitro were analyzed.The experimental results showed that the optimum enzymolysis technology of bovine bone polypeptide was as follows:the mixing ratio of neutral protease and trypsin 1:4,the enzyme dosage 1.4％,the enzymolysis temperature 39.6 ℃,and the enzymolysis time 2.1 h.Under this enzymolysis condition,the yield of bovine bone polypeptide was 17.11％±0.24％.Bovine bone peptides were treated by membranes with different pore sizes to obtain three kinds of small molecular weight peptides,which were more than 5 000 Da(polypeptide Ⅰ),600-5 000 Da(polypeptide Ⅱ)and 150-600 Da(polypeptide Ⅲ).The antioxidant activities of peptides with different molecular weights and enzymatic hydrolysate of bovine bone were tested with DPPH radical scavenging rate,hydroxyl radical scavenging rate and reducing power as indicators.The results showed that all samples had certain antioxidant activities,among which the activity of polypeptide Ⅲ with molecular weight of 150-600 Da was better than other samples,and its DPPH radical scavenging activity,hydroxyl radical scavenging activity and reducing power at the concentration of 10 mg/mL were 87.89％±0.18％,52.49％±0.22％and 0.382±0.02,respectively.
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