首页|黑果腺肋花楸多酚提取工艺优化及其抗氧化性研究

黑果腺肋花楸多酚提取工艺优化及其抗氧化性研究

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黑果腺肋花楸中富含多酚类物质,多酚具有多种生物活性,提取其中的多酚可应用于食品加工.通过超声波辅助提取黑果腺肋花楸中的多酚类物质,采用单因素试验确定了三个显著因素,分别是料液比、温度、pH.由响应面试验得到了优化后的提取工艺,并且进一步研究了多酚的抗氧化能力.最佳提取工艺为料液比1∶53(g/mL)、温度34 ℃、pH 6、提取时间25 min,黑果腺肋花楸多酚得率为0.84 mg/g.黑果腺肋花楸多酚对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2'-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)自由基、羟自由基的清除率分别为97.59%,89.41%和81.56%,表明黑果腺肋花楸多酚具有较好的抗氧化性.
Optimization of Extraction Technology and Properties of Polyphenols from Aronia melanocarpa and Study on Antioxidant Activity
Aronia melanocarpa is rich in polyphenols,which have various biological activities.Extracting polyphenols from it can be applied in food processing.By ultrasonic-assisted extraction of polyphenols from Aronia melanocarpa,three significant factors were determined by single factor test,which were solid-liquid ratio,temperature and pH.The optimized extraction process was obtained by response surface test,and the antioxidant capacity of polyphenols was further studied.The optimal extraction process was as follows:solid-liquid ratio 1∶53(g/mL),temperature 34 ℃,pH 6,and extraction time 25 min,and polyphenol yield was 0.84 mg/g.The scavenging rates of the polyphenols of Aronia melanocarpa on the free radicals of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),2,2'-hydrazine bis(3-ethyl Benzothiazole line-6-sulfonic acid)diamine salt(ABTS)and hydroxyl radical were 97.59%,89.41%and 81.56%,respectively,indicating that the polyphenols of Aronia melanocarpa had good antioxidant properties.

Aronia melanocarpapolyphenolsresponse surfaceantioxidant capacity

王亚菲、杜琳、潘勇韬、朱凤妹

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河北科技师范学院(秦皇岛市 066000)

秦皇岛市林业局(秦皇岛市 066000)

黑果腺肋花楸 多酚 响应面 抗氧化能力

河北省"三三三人才工程"资助项目

B20221006

2024

食品工业
上海市食品工业研究所

食品工业

影响因子:0.47
ISSN:1004-471X
年,卷(期):2024.45(1)
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