Development of a Rapid Determination Strip for Diethylstilbestrol and Its Application in the Determination of Poultry Meat
Develop a rapid determination strip for diethylstilbestrol applicated in the determination of poultry meat.With the application amount of potassium carbonate,concentration of C-line and T-line,the best parameters needed in the experiment were determined.A rapid method for the determination of diethylstilbestrol in poultry meat by colloidal gold was established.The formula of detection working solution was determined by single factor and orthogonal test.The results showed that the content of BSA 0.2％and trehalose 0.2％in the working solution was determined by single factor test.The content of NaCl 0.2％,casein 0.25％and ethanol 1.0％in the working solution was determined by orthogonal test.The detection limit,sensitivity and cross reaction rate of the test strip were investigated.The test result showed that the detection limit of test paper strip was 1 μg/kg,the sensitivity was 100％,the cross-reaction rate of diethylstilbestrol and diethylstilbestrol was 33.3％,and the cross-reaction rate of estradiol benzoate and ethinyl estradiol was less than 0.1.It could be concluded that all of the parameters of detection requirements complied with the detection requirements,and it could be used as a screening method in practice.
万方数据
维普
