Quantitative Analysis of Sodium Benzoate in Beverages Based on 1H-NMR
A 1H-NMR method was developed for the quantification of sodium benzoate in beverages and oral liquids.The hydrogen spectra of the samples were collected by using noesygpprld nuclear magnetic pulse sequence to suppress the water peak,and using pyrazine as the internal standard.Compare the response peak area of the internal standard and sodium benzoate quantitative peak to calculate the concentration of sodium benzoate in the samples.The plot of the peak area integral value versus concentration of sodium benzoate couold be used to obtain the linear regression equation Y=4.247 3X+0.003 5,and the correlation coefficient(r)was calculated to be 0.999 9 in the range of 0.015-2 g/L,which indicated that this method had good linearity.The SLOD and SLOQ values were determined to be 1.03×10-2 g/L and 3.11×10-2 g/L,respectively.The relative standard deviation of 6 parallel determinations was 0.77％,and the recoveries of the three concentrations were 98.06％-99.93％.The content of sodium benzoate in 19 kinds of beverages and oral liquids was determined by the established method.The relative deviation between the results obtained by the 1H-NMR and the high-performance liquid chromatography was less than 5％,which proved the feasibility and accuracy of this method.The 1H-NMR method established was simple to operate,and could determine the content of sodium benzoate quickly and accurately in beverages and oral liquid.This method could provide a reference for production enterprises and regulatory authorities.
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