The method of ultra-high performance liquid chromatography mass spectrometry and liquid chromatography for the determination of vitamin D3 in infant formula foods is optimized.Samples are diluted and redissolved in large quantities,while small quantities are quickly preprocessed in batches.There is a comparative study on the equivalence between ultra-high performance liquid chromatography coupled with mass spectrometry and ultra-high performance liquid chromatography.The Vitamin D3 shows a good linear relationship in the range of 20.0-400.0 μg/L by LC-MS/MS,the Vitamin D3 shows a good linear relationship in the range of 50.0-1 000.0 μg/L by HPLC,and the correlation coefficient R2 is more than 0.998.The limit of quantitation of LC-MS/MS method is 0.879 μg/100 g,which is below the limit of quantitation of HPLC method(1.35μg/100 g).The recovery rate of Vitamin D3 by LC-MS/MS is in the range from 88.0％to 97.6％,with the precursorions(n=6)in the range of 4.8％to 3.0％,and the recovery rate of Vitamin D3 by HPLC is in the range from 89.8％to 100.3％,with the precursorions(n=6)in the range of 2.0％to 3.8％.The optimized detection method has good sample representativeness,simple operation,and can be applied to all categories of infant formula food.The two methods have equivalence.
关键词
婴幼儿配方食品/维生素D,/方法等效性/前处理优化
Key words
infant formula food/vitamin D3/method equivalence/improvement for preparation steps
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