金属离子对枯草芽孢杆菌发酵产γ-聚谷氨酸的影响
Effect of Metal Ions on γ-Polyglutamic Acid Production Fermented by Bacillus subtilis
马双双 1张英华 1张杰 1李珍爱 1李艳艳 1李海军2
作者信息
- 1. 山东福瑞达生物科技有限公司(临沂 276715)
- 2. 山东福瑞达生物科技有限公司(临沂 276715);山东福瑞达医药集团有限公司(济南 250098);山东省药学科学院(济南 250098)
- 折叠
摘要
γ-聚谷氨酸(γ-PGA)发酵黏度大、产能小,直接影响γ-PGA的推广应用.为提高γ-PGA的发酵产量,旨在探究金属离子对枯草芽孢杆菌发酵产γ-PGA的影响,以自行选育的枯草芽孢杆菌FRD215为出发菌株,采用单因素试验和正交试验,探讨6种金属离子对枯草芽孢杆菌发酵产γ-PGA的影响.摇瓶试验结果表明,钙、锰、铁离子可促进枯草芽孢杆菌产γ-PGA,钠、铜、锌离子对枯草芽孢杆菌产γ-PGA无明显影响.在发酵培养基中同时添加0.8 g/L CaCl2、0.02 g/L FeCl3·7H2O和0.1 g/L MnSO4·H2O,γ-PGA产量达53.34 g/L,比优化前至少提高40.0％以上.试验结果对枯草芽孢杆菌FRD215大规模生产和应用具有指导意义.
Abstract
The high viscosity and low yield of γ-polyglutamic acid fermentation limited its application.In order to increase the production of γ-polyglutamic acid,the effect of metal ions on γ-polyglutamic acid production by Bacillus subtilis was studied.The effects of six metal ions on γ-polyglutamic acid production by Bacillus subtilis FRD215 were studied by single factor experiment and orthogonal experiment.The results of flask-shaking experiment showed that calcium,manganese and iron ions could promote the production of γ-PGA by Bacillus subtilis,but sodium,copper and zinc ions had no significant effect on the production of γ-PGA by Bacillus subtilis.The addition of 0.8 g/L CaCl2,0.02 g/L FeCl3·7H2O and 0.1 g/L MnSO4·H2O to the fermentation medium concurrently resulted in a γ-PGA yield of 53.34 g/L,and a significant 40.0％increase from the prior optimization.The results have guiding significance for large-scale production and application of Bacillus subtilis FRD215.
关键词
枯草芽孢杆菌/γ-聚谷氨酸/金属离子Key words
Bacillus subtilis/γ-polyglutamic acid/metal ions引用本文复制引用
出版年
2024
NETL
NSTL
万方数据