Fingerprinting of Rosa roxburghii Tratt and Rosa sterilis Based on GC-MS and UPLC Methods
With Rosa roxburghii Tratt and Rosa sterilis as the research object,using fingerprinting technology to compare the volatile and water-soluble components of Rosa roxburghii Tratt and Rosa sterilis,explore the differences and similarities of the two main chemical compositions.Five batches of Rosa roxburghii Tratt and five batches of Rosa sterilis were selected for the experiment,their volatile components were analyzed and compared by steam distillation combined with gas chromatography-mass spectrometry(GC-MS),and their water-soluble components were analyzed and compared by methanol ultrasonic extraction method and UPLC method.While similarity evaluation and cluster analysis methods were used to study the fingerprint profiles of five batches of Rosa roxburghii Tratt and five batches of Rosa sterilis,GC-MS and UPLC fingerprints of five batches of Rosa roxburghii Tratt and five batches of Rosa sterilis were established,and five common peaks were established.The compositions of Rosa roxburghii Tratt and Rosa sterilis varied considerably,and the samples differed to some extent between different origins and within the same origin.The results compared the differences between the volatile and water-soluble components of Rosa roxburghii Tratt and Rosa sterilis,which provided certain theoretical basis for the study of dietary supplementation,therapeutic material basis and quality control,and meanwhile were of great significance to the production and cultivation of Rosa roxburghii Tratt and Rosa sterilis,processing and development,and quality control.
Rosa roxburghii TrattRosa sterilisfingerprinting technologygas chromatography-mass spectrometry(GC-MS)ultra-performance liquid chromatography(UPLC)
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