基于磁性分子印迹技术检测食品中利巴韦林含量
A Detected Method of Ribavirin in Food Based on Magnetic Molecular Imprinting Technique
金文丽 1周鑫魁 2李建民 1张连钢 1张婧蕾 1袁嘉男1
作者信息
- 1. 嘉峪关市食品药品和医疗器械检验检测中心(嘉峪关 735100)
- 2. 甘肃省商业科技研究所有限公司(兰州 730010)
- 折叠
摘要
建立一种针对食品中利巴韦林快速、高效、准确、易操作的检测方法.利用自主合成的磁性分子印迹材料,作为固相分散萃取填料,不需要固相萃取柱和浓缩步骤即可完成食品中利巴韦林含量检测试验.制备的利巴韦林磁性分子印迹微球(MMIPs),平均粒径2.5 μm,粒径分布均匀,单分散性良好,有利于目标物的吸附和洗脱.1 min内达到吸附平衡,最大吸附容量5 835 mg/kg.利用MMIPs制备了固相分散萃取填料,用于实际样品检测过程中.在中性条件下用乙腈作为提取液,固相分散萃取填料净化杂质,富集目标物,通过液相色谱对目标物进行定量检测.在加标质量分数为0.5,2.0和10.0 mg/kg条件下,方法回收率为80.6％～90.2％.精密度SRSD=4.38％(n=9),色谱定量重复性SRSD=2.60％,定性重复性SRSD=0.22％.方法检出限为0.02 mg/kg,定量限为0.05 mg/kg.待测液质量浓度为0.1～50.0 μg/mL,即样品质量分数0.02～10 mg/kg范围内呈良好线性,线性回归方程y=541.33x+12.092,相关性系数R2=0.999 8.该方法和原理可以为磁性分子印迹材料应用领域提供一定的参考和实验思路.
Abstract
Create a fast,efficient,accurate and easy-operated detection method of strong adsorption capacity for ribavirin.The magnetic molecule-imprinted material can be used as a solid phase dispersed extraction filler to detect ribavirin in food products instead of solid phase extraction column or concentration steps.Ribavirin magnetic molecularly imprinted polymers(MMIPs),including an average particle size of 2.5 μm,an uniform particle size distribution and a good monodispersion,are synthesized independently as a solid phase dispersed extraction filler,which can effectively adsorb and purge the target substance.The maximum adsorption capacity of MMIPs is 5 835 mg/kg,and the adsorption equilibrium time is 1 min,which can quickly and effectively adsorb the target substance in the sample solution.Under neutral conditions,acetonitrile is used as extraction liquid,solid phase dispersion extraction filler is used for purifying impurities and enriching target substance,and quantitative detection of target substance is carried out by liquid chromatography.The recovery rate is 80.6％-90.2％,the precision SRSD is 4.38％,the quantitative repeatability is 2.60％,the qualitative repeatability is 0.22％,the detection limit is 0.02 mg/kg,the quantitation limit is 0.05 mg/kg.The linear regression equation is y=541.33x+12.092,and the correlation coefficient is R2=0.999 8.It is suitable for detection of Ribavirin in food products.The method and principle can provide some reference and experimental ideas for the application of magnetic molecularly imprinted materials.
关键词
利巴韦林/磁性分子印迹/微球/液相色谱/固相分散萃取Key words
ribavirin/magnetic molecular imprinting/microspheres/liquid chromatography/solid phase dispersion extraction引用本文复制引用
出版年
2024
NETL
NSTL
万方数据